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Sunitinib has also been shown in tumor-bearing mice and patients with metastatic renal carcinoma to lower the number and efficacy of Tregs [89]

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Finally, sections were washed and incubated with Hoechst 33342 nucleic acid stain (Invitrogen, #H1399), washed in ddH 2 O, mounted with Fluoromount-G (Southern Biotech, #0100-01), and stored at 4 C in the dark

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We are talking about a fundamental biological necessity