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These shRNAs were in the pLKO.1 vector (shACTL6A-1 and shACTL6A-2)

A serial dilution series of limiting concentrations of complexes were incubated with 2 nM of a radiolabelled target RNA, in 18 mM HEPES pH 7.4, 100 mM NaCl, 1 mM MgCl 2 , 2.5% (v/v) glycerol, 0.003% (v/v) IGEPAL CA-630, 0.5 mM BME, 0.017 mg ml 1 recombinant albumin and 0.003 mg ml 1 yeast tRNA, at 37 C for 1 h

The primary outcomes were the percentage weight change and the proportion of participants losing at least 5% of their baseline weight, using treatment-policy estimand analysis (intention-to-treat principle)