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After pretreatment, infection, or transfection, cell medium was exchanged for medium containing lethal compounds and 0.022 M of the viability dye SYTOX Green (SG, Cat
The samples were centrifuged and supernatants were evaporated under a stream of N 2 , before reconstituting in ultrapure water
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Add the water gently down the vial wall, do not shake, and discard if the solution is cloudy
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