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Cell viability was measured by CellTiter 96 AQueous one solution cell proliferation assay system (Promega) according to the instructions of the manufacturer

This heightened oxidative environment not only damages DNA but also promotes lipid peroxidation and protein oxidation, which can further disrupt cellular signaling pathways and contribute to carcinogenesis [255]

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Two independent investigators systematically searched Web of Science, PubMed, and Cochrane Library and extracted data from 87 studies with 4928 ASD children and 4181 healthy control (HC) children

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