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To further characterize the feature of new OL genesis during whole course of this model, we further took the advantage of our recently identified OL lineage cell stage-specific marker, ENPP6, a choline-specific ecto-nucleotide pyrophosphatase /phosphodiesterase (ENPP), which is highly expressed in newly-forming/formed OLs and at a much lower levels in these more mature/old (earlier-formed) myelinating OLs (to the extent that the ISH signal would easily disappear if the development time were shortened), but not at all in OPCs, neurons, astrocytes or vascular endothelial cells (Xiao et al., 2016

doi:10.1093/toxsci/kfi057

Eur J Clin Pharmacol 31 : 217222

major targets is presented in Tables 1 and 2, the list of 254 FDA-approved drugs (henceforth referred to as 'Lm254') is presented in Additional file 1: Table S2, and the list of all drugs associated with the 8 synthetic lethal targets is presented in Additional file 1: Table S3

Prior to sacrifice, blood was collected from the inner canthus vein with a lancet into heparin-coated tubes

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