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After incubation at 37 C with shaking (500 rpm) for 1 h., the cells were pelleted at 5,000 x g for 10 min at 4 C, washed once with ice-cold phosphate-buffered saline (PBS, pH 7.4), resuspended in 200 L of cold TE lysis buffer (30 mM TrisHCl, 1 mM EDTA, pH 8.0), and transferred to Lysing matrix B tubes (MP Biomedical)

The exchange of hydrogen nuclei by deuterium nuclei in metabolites after the administration of [6,6- 2 H 2 ]-labeled glucose can be detected indirectly using proton MRS

Truong MT , Munden RF

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