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Upon T cell activation, both the transcript and enzymatic activity of the cytosolic isoform of branched chain aminotransferase (BCATc) are rapidly induced, leading to the production of the ketoacid -ketoisocaproate

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SERCA activity is also maintained by the ER lectin chaperone calnexin, which maintains a basal level of oxidation of SERCA that is required for its activity

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( In vitro experiments demonstrated that NNMT overexpression significantly increased Sirt1 protein expression in primary hepatocytes, while NNMT knockdown led to a notable decrease in Sirt1 protein expression