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Funding The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: this work was funded by the Ministry of Science, Technology and Innovation (COLCIENCIAS), Grant no

Complementary, this finding was confirmed by flow cytometry (flow cytometry plots: Fig

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Over longer periods of time, prolonged incubation of the receptors with isoproterenol was associated with a 5060% decrease in the number of 2 AR binding sites in the plasma membrane and a pool of sequestered receptors in vesicles that were uncoupled from adenylyl cyclase [8]