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Beraja, DO, MS, OhioHealth Doctors Hospital, USA

The tissue culture method provides a smaller total protein yield of EVs, but it shows a higher particle: protein ratio than the enzymatic digestion method, indicating higher EV purity

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However, several methods have been recently developed that allow observations of large-scale domains, such as enlarging organelles through hypotonic swelling or fatty acid feeding (Shen et al., 2017