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At visits 1 and 5, neurocognitive assessments (Trail Making Test, Block Corsi Test, Subtest Digit Span, Category (Animal) Fluency and Controlled Oral Word Association Test, COWA (FAS test), and Rey Auditory Verbal Learning Test), collection of blood samples, measurement of abdominal circumference and body mass index (BMI) were carried out

Global protein abundance profiling by mass spectrometry Sample preparation (A) Flash frozen liver sections (~25 mg) from WT or Gclc / mice (3 biological replicates each per TMT sample) were thawed on ice and resuspended in DPBS, supplemented with protease (cOmplete, EDTA-free protease inhibitor cocktail, Roche, #11873580001) and phosphatase (PhosSTOP, Roche, #4906845001) inhibitor tablets

TAMs shape ferroptotic sensitivity by controlling iron transport, redox buffering, glutathione metabolism, lipid peroxidation pathways, and mitochondrial stress signaling

it's about maximising the percentage of glutathione that reaches adipose tissue, liver mitochondria, and endothelial cells during the metabolic window when rapid fat oxidation is generating the most reactive oxygen species (ROS)

The next major upgrade is learning how to dissect experimental MCAT figures under time pressurebut that is a separate skill set, and a separate conversation.