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For example, due to the downregulation of genes for purinergic receptors (e.g., P2ry12 ) and potassium leak channels (e.g., Thik-1 ) in aged mice, the ability for microglia to sense hyperactive neurons and migrate toward them in order to facilitate rapid repolarization would be reduced, impairing their beneficial modulation (Hickman et al., 2013

Amano H, Chaudhury A, Rodriguez-Aguayo C, Lu L, Akhanov V, Catic A, Popov YV (2019) Telomere dysfunction induces sirtuin repression that drives telomere-dependent disease

Cell pellets were resuspended in 100 L lysis buffer (10 mM EDTA, 2 mg/mL lysozyme, 20 mM Tris-HCl, 10 mM IAM, pH 8.0) and incubated for 30 min on ice

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J Urol 1983;129:907912

pylori , upregulating ZO-1 and lipid rafts to increase epithelial integrity, inhibiting IL-8 release, reducing neutrophil infiltration, and promoting macrophage clearance of apoptotic cells to rapidly repair mucosal damage