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The starting conditions were 65% A, optima grade water with 0.1% formic acid and 2 mM ammonium formate, and 35% B, optima grade methanol with 0.1% formic acid and 2 mM ammonium formate

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This structure stabilizes the complex and supports its role as a redox-inactive carrier of bioavailable copper (Pickart, Vasquez-Soltero, and Margolina 2012)