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10% fetal bovine serum/FBS (Life Technologies 10270106) and 1% penicillin/streptomycin solution (P/S, Sigma-Aldrich P0781) were used for cell culture, except for DHODH knockout cells, which were cultured with the additional supplement of 50 M uridine at 37 C in a humidified incubator containing 5% CO 2

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doi: 10.1111/bcp.12070 181 EricksonCAWinkLKRayBEarlyMCStiegelmeyerEMathieu-FrasierLet al

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Bene MC, Faure G, Bordigoni P et al (1982) In vitro induction of monoclonal antibody-defined T-cell markers in lymphocytes from immunodeficient children by synthetic serum thymic factor (FTS)