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Lysis was performed in 3 cycles of vortexing for 30 s, incubation in liquid nitrogen for 1 min followed by subsequent thawing at room temperature and sonication for 10 min in an ice-cold water bath

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Treatment (intraperitoneal injection) with five doses of methanolic extract for Black seed was found to enhance the total white blood cells count [up to 1.210 4 cells/mm 3 ]

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[DOI] [PubMed] [Google Scholar] Tanno M, Kuno A, Yano T, Miura T, Hisahara S, Ishikawa S, Shimamoto K, Horio Y

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