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Early work established that UreG, UreF and UreD form a 2-fold symmetric UreG 2 F 2 D 2 complex in which the UreG dimer fits snugly between an approximately linear UreD-UreFUreF-UreD complex with UreG 2 docked opposite the UreF 2 core, positioning the subunit-bridging Ni II ion at the UreG 2 F 2 interface (Figure 40A)

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