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Annexin-V staining, imaging, and quantification DRG explants seeded on glass bottom dishes (CellVis) were incubated with 1 g/ml Annexin-V (AAT Bioquest) in annexin-V buffer (10 mm HEPES/NaOH, pH7.4, 140 mm NaCl, and 2.5 mm CaCl 2 ) for 15 min at RT

Common signs are: Fatigue and weakness Neurological changes, such as numbness or tingling in extremities Megaloblastic anemia, characterized by large, abnormal red blood cells We urge quick medical help if these signs dont go away

Diabetes Res Clin Pract 164:108183

in late-stage type 2 diabetes, pancreatic beta cell dysfunction may necessitate exogenous insulin administration (Mayo Clinic 2014

Cell 179, 10331055

A., Peana A
