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NO production results in suspension-induced muscle atrophy through dislocation of neuronal NOS

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Beads were washed three times in cold KPBS, then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10min at 4C

10.1016/j.ctrv.2014.02.006 181 NishidaN.YamashitaS.MimoriK.SudoT.TanakaF.ShibataK.et al (2012)