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Since the liver is a major metabolic organ, liver disease is associated with metabolic diseases, such as obesity, insulin resistance, type 2 diabetes (T2DM), and cardiovascular disease

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Cell Metabolism , 21(3), 443-454

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Western blot The specimens were lysed in radio immunoprecipitation assay buffer (RIPA buffer) (Cyto matin gene, Iran) with a phenyl methyl sulfonyl fluoride (PMSF) protease inhibitor (Sigma, USA) and centrifuged (15,000 rpm, 15 min, 4 C)