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Reactive species assays Reactive species were measured using the luminescent probe L-012 (Wako Chemical) and the fluorescent dye fluorescein-boronate (Fl-B) 63

These findings highlight the capacity of microbiome metabolite-enriched LNPs to enhance mRNA vaccination effectiveness by improving adaptive immunity and immunological memory [350,351,352]

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The mixture was centrifuged at 12,000 rpm at 4C for 5 min, and the supernatant was collected and used to determine the amount of the compounds and substrates using LC-MS

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