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The cryosections were washed with PBS and treated with methanol for 10 min, and then four times with 0.2% PBT (0.2% Triton X100 in PBS) for 10 min each time, and finally with Image-iT FX Signal Enhancer (Thermo Fisher Scientific) for 30 min

Am J Geriatr Pharmacother

332 These differences are greater than can be explained by analytical differences between methods

( f ) Spectroscopic intermediate measured at 77 K after illumination (530 nm) at 190 K for 15 min and incubation in the dark for 15 min at 260 K (red curve) and spectrum of the Tt CBD-H132A mutant light state measured at room temperature after illumination (530 nm) until complete photoconversion (black curve)

Furthermore, nanomedicine presents opportunities for neuroprotective strategies by employing antioxidant and anti-inflammatory NPs to protect and repair damaged neurons

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