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Isolation and Structure Determination of a Candidate Tetradepsipeptide Ethyl Ester ( m/z 453) and Octadepsipeptide Ethyl Ester ( m/z 837), Respectively The combined EtOH extracts obtained from cultures (100 mL) of strain F4810/72 were concentrated to about 25 mL in vacuum and, after dilution with H 2 O (1/10, v/v), aliquots (5 mL) were separated by means of preparative RP-HPLC on a PrepStar system (Varian, Darmstadt, Germany) consisting of two HPLC-pumps (Model SD-1), a two-wavelength UV detector (Prostar 325), a fraction collector (Model 701) and equipped with a 250 21.2 mm, 4 m, 90A, Jupiter Proteo column (Phenomenex) as the stationary phase

Pharmaceuticals (2021) 14

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Other side effects reported ranged from lipohypertrophy at injection site, papilloedema (related to cranial hypertension), and facial nerve paralysis [276, 285], however symptoms did not prevail after treatment pause and restarting with lower doses [268]