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I plan to get my second dose on schedule, at 4 weeks

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Sucrose fractionation Quadriceps (2.4 mg) pooled from five animals of each group were homogenized with a Dounce homogenizer in 1 mL polysome extraction buffer (PEB): 20 mM TrisHCl pH 7.5, 100 mM KCl, 5 mM MgCl 2 , 0.3 % NP-40, 60 U/mL RiboLock RNase Inhibitor (Thermo Fisher Scientific), 100 g/mL cycloheximide, 5 M FAD, cOmplete EDTA-free protease inhibitor cocktail and phosphatase inhibitor cocktail (Sigma-Aldrich)

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