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Protein misfolding, functional amyloid, and human disease

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Expression of ElF2, a major regulator of the ISR pathway, is increased in oligodendrocytes in active MS lesions compared to levels in normal appearing white matter or control tissue 42

For example, glutathione becomes rapidly oxidized to glutathione disulfide either by glutathione peroxidase (GPx) or by enzyme-independent chemical reactions [102]

These compounds can decrease the rate of NO production to constitutive NO production levels (105, 106)

After centrifugation at 65,000 g for 30 min, the supernatant was collected and incubated with 10 ml Ni-NTA resin (Cytiva) in the presence of 8 mM imidazole to prevent the nonspecific binding at 4 C overnight