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CMFDA has been frequently used to monitor glutathione in both animal and plant systems (Voehringer et al., 1998

Plant Physiol Biochem 70:204212 Robbins ME, Cho HY, Hansen JM, Luchsinger JR, Locy ML, Velten M (2021) Glutathione reductase deficiency alters lung development and hyperoxic responses in neonatal mice

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Standards or samples are added to the appropriate microtiter plate wells, followed by a biotin-conjugated antibody specific to GPX1 (Glutathione Peroxidase 1)

The figures below show the direct and indirect human exposure to whale derived products from the hump back whale and sperm whale

This step only needs to be done once per vial
