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The concentration of methane produced during the experiment was determined using gas chromatography (GC, Agilent Technologies 7890B)

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In our study, SULT2B1a wild-type and all selected allozymes have been analyzed for sulfating activity toward cholesterol

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GSH/GSSG-Glo assay GSH/GSSG ratio of CuCl 2 -treated HepG2 and HepG2 ATP7B-KO cells was measured by GSH/GSSG-Glo Assay (Promega) according to manufactures instructions

based on this, a recommended intake (PRI) of 1.9 mg/day was proposed