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doi: 10.1172/JCI18058

Transient transfection of the PX459-sgRNA into the target cancer cell lines was performed using the Lipofectamine 3000 Transfection Reagent (Invitrogen) in 6-well plates (Corning Falcon) followed by puromycin selection (Gibco, 15 g/ml) for 3 days

The presence of eosinophils within white adipose tissue (WAT) provides protection against obesity, promotes thermogenic energy expenditure and metabolic homeostasis in mice and this is, at least in part, due to their production of IL-4, which acts to promote the alternative activation of macrophages (see reviews by 60,61 )

Methyl metabolism is ancient and well conserved from bacteria to humans

Many personal care products are laden with endocrine-disrupting chemicals and heavy metals that can be deleterious to our health from constant exposure

Miniaturized imaging systems