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MK-2206, an allosteric inhibitor of AKT, a downstream target of PI3K, might have effects on inflammatory pathways relevant to emphysema

Bovine serum albumin (BSA-50, Rockland) was used as a negative control

Nuclear factor erythroid 2-related factor 2 (NFE2L2 or NRF2), a basic leucine zipper transcription factor that binds antioxidant response elements (AREs) present in the promoter of several antioxidant enzymes, is a crucial regulator of the oxidative stress response

Long-term HFD consumption increased hepatic IKK- glutathionylation and NF-B suppression The mechanistic investigations in our cell culture studies provided evidence suggesting that intracellular GSSG accumulation sensitizes hepatocytes to TNF cytotoxicity via the induction of IKK- glutathionylation, leading to reduced IKK- phosphorylation and subsequent NF-B suppression