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After 5 days, residual microglial cells were eliminated by treatment with 50 mM L-leucine methyl ester (Merck Millipore, #L1002) for 1 h, and microglia-free astrocytes were cultured for a further 7-9 days

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ROS, often labeled as free radicals, are highly reactive and can cause cellular damage

Anxiety 2023, 112

doi: 10.1177/0706743720916356, PMID: [DOI] [PMC free article] [PubMed] [Google Scholar] Shallcross J., Wu L., Wilkinson C

Mechanical stress can break down the peptide structure, reducing its efficacy and shortening how long GHK-Cu Cosmetic vial lasts