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Determination of Intracellular NAC and PQ Concentration with Ultraperformance Liquid Chromatography (UPLC) The intracellular levels of NAC, PQ, and GSH were determined by an ultraperformance liquid chromatographic (UPLC) method using a Waters Acquity system equipped with a binary solvent manager, an automated sample manager, and a photodiode array detector (Waters, Milford, Mss, USA) as described previously by Mitsopoulos and Suntres [26]

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Lactate production was reduced initially, however, six hours after exposure, the lactate level in the exposed lenses was greater than contralateral lenses (167)

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