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Aging Dis 12:3641

Sometimes this issue is fixed simply by optimizing buffers, for instance by increasing salt or detergent concentration, so that the interacting protein no longer binds to the GST only control, but still binds to your GST-fusion protein of interest

After washing twice with UA as above, 100 L of 50 mM AmBic (50 mM NH 4 HCO 3 in water) was added to the filter unit and the filter was centrifuged at 14,000 g for 10 min