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After incubation, the sections were washed four times in PBS-Triton for 10 min each, followed by incubation with the secondary antibody, Alexa Fluor 488 donkey anti-rabbit IgG (1:200, AntGene, China), for 2 h at room temperature

Lu L, Wang JR, Henderson YC, Bai S, Yang J, Hu M, Shiau CK, Pan T, Yan Y, Tran TM, Li J, Kieser R, Zhao X, Wang J, Nurieva R, Williams MD, Cabanillas ME, Dadu R, Busaidy NL, Zafereo M, Navin N, Lai SY, Gao R

The mass spectrometer was interfaced with the liquid-chromatography system using an electrospray ion source

Sutton et al., 2012

Improved skin quality

Hence, the safety associated with copper delivery through the skin needs to be established