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J Infect Dis 203(8):11361146 Nathan C, Shiloh MU (2000) Reactive oxygen and nitrogen intermediates in the relationship between mammalian hosts and microbial pathogens

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[DOI] [PMC free article] [PubMed] [Google Scholar] 51.Jiang Y., Yang M., Sun X., Chen X., Ma M., Yin X., Qian S., Zhang Z., Fu Y., Liu J., et al

Adding protease inhibitors such as 1mM PMSF to cell lysates does not inhibit binding or elution of polyhistidine-tagged proteins with the HisLink Resin and is highly recommended to prevent degradation of the protein of interest by endogenous proteases