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[DOI] [PMC free article] [PubMed] 34.Coleman J., Green P.J., Inouye M

These data indicate that activation of Htr2a + neurons by serotonin may have an anxiolytic effect by suppressing innate fear responses, consistent with the observation that reduced levels of serotonin in the amygdala are associated with anxiety in humans 67

Yes, neurological damage from B12 deficiency can become irreversible if treatment is delayed, even though haematological abnormalities typically resolve with supplementation