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Samples were run on either a SeQuant ZIC-pHILIC LC column, 5 m, 150 4.6 mm (MilliporeSigma, Burlington, MA) with a SeQuant ZIC-pHILIC guard column, 20 4.6 mm (MilliporeSigma, Burlington, MA) or an Atlantis Premier BEH Z-HILIC VanGuard FIT column, 2.5 m, 2.1 mm 150 mm (Waters, Milford, MA)

J.PigoniM.PaulsenB.AdiconisX.KimK.et al (2022)

The number of repeats in UAS -( G 4 C 2 ) 9 or 42 transgenic flies were determined by genomic PCR using the forward (5-AACCAGCAACCAAGTAAATCAAC-3), and reverse (5-TGTTGAGAGTCAGCAGTAGCC-3) primers, which amplifies a part of the UAS -( G 4 C 2 ) n sequence, including G 4 C 2 repeat sequence, followed by sequencing using the forward (5-GCCAAGAAGTAATTATTGA-3) and/or reverse (5-TCCAATTATGTCACACC-3) primers

The hypothesis holds that a changed set point persistently pushes the organism towards a pathological dysfunctional state which fails to reset

Brudno, J

21 The sequences of primers used in this study are listed in Table 1