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Interim Summary of Conclusions and Dietary Recommendations on Total Fat & Fatty Acids

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2007;130(Pt 8):2037-2044

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Previous work in bison reported that offering a choice of feeds (ad libitum choice of whole corn, wheat-middlings, alfalfa hay and/or oat hay) and additional space to roam in loose confinement (334 m 2 /animal) improved average daily gain (ADG) when compared to a more traditional feedlot set-up (21 m 2 /animal) and standardized total mixed rations [54]

Fluorescence staining with labeled antibodies, phalloidin and dextran For immunostaining, animals were decapitated in 1x PBS on a poly-l-lysine-coated glass slide followed by mounting a coverslip on top, removing excessive liquid and freezing the sample in liquid nitrogen