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however, its strong hydrophilicity and poor lipid solubility result in limited bloodbrain barrier (BBB) permeability and suboptimal clinical performance

Its particularly effective in environments with reduced sunlight

Seeman P, Guan HC, van Tol HHM

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At varying time points (0, 2, 5, 15, 30 min), purified Pt GSTF8 (8 M) and GSH (20 M) were added to an aliquot of the equilibration reaction and the resulting mixtures were incubated at 30 C for 30 min at 1200 rpm using a 3 mm shaking diameter before extraction with 2 vol of 75% methanol in H 2 O, 1 mM EDTA, 0.3% hydrochloric acid

This procedure equally distributes the error among the reaction rates of the static module (Yugi et al., 2005)