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Bacterial isolates with slow growth rates often require cultivation for 710 days, followed by 12 rounds of purification lasting at least 14 days

According to Joo et al

The microbiota regulates the expression of tight junction proteins (such as ZO-1 and occludin) in intestinal epithelial cells by releasing specific metabolites and signaling molecules, thereby safeguarding the structural and functional integrity of the intestinal barrier ( Studies demonstrate that when TLR5 / germ-free mice are infected with adherent-invasive Escherichia coli (AIEC) and subsequently exposed to a specific SPF microbial environment, they develop colitis symptoms ( The gut microbiota of IBD patients exhibits a trend towards reduced diversity, manifested explicitly by a decrease in the abundance of commensal Clostridia and a significant enrichment of pathogenic bacteria