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Other pathogenic species that target TJ protein complexes to disrupt gut barrier integrity include Vibrio cholerae and Aeromonas hydrophila which secrete a hemagglutinin/protease and a pore-forming aerolysin, respectively, facilitating the direct cleavage of occludin (Nava et al

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These are important differentiations

Possible causes include: Incorrect solvent selection Incompatible pH Hydrophobic aggregation Precipitation Cloudiness should not automatically be treated by additional shaking or vortexing