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For the comet assay experiments, cells were seeded at a density of 2 10 5 cells/ml per well in 24-well plates and cultured for 24 h prior to treatment

Nat Commun 2021, 12 (1), 4347

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Glutathione injections work by clearing the body of toxins and free radicals that lead to hyperpigmentation, age spots, sun spots, wrinkles, acne, and even melasma

Furthermore, ACA suppressed the expression of Th2 cytokines (IL-4, IL-6, and IL-13) and Th1 cytokines (IL-12 and IFN-) in the ACA-treated group in a dose-dependent manner

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