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Inject the concentrated, filtered sample onto an appropriate gel filtration column equilibrated in PBS or other compatible sample buffer

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While the biological relevance of this method in honey bees it is not yet knownit has yet to be determined if seminal fluid components naturally exit the reproductive tract of honey bee queensinjecting seminal fluid components into the hemocoel is a well-established method for examining post-mating changes in many other insects 78,79,80,81,82,83,84,85,86,87

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Furthermore, the investigators focused on providing scientific evidence for the neuroprotective qualities of RES against PD and outlined the mechanism underlying therapeutic development considerations