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coli cells were washed with the PBS buffer for three times at 4C, and suspended in the 5 ml PBS solution, respectively
R., Ditzel H

doi: 10.1016/j.cell.2007.09.037

Target RNA modification The target RNA was first folded by adding 1 PKN buffer (20 mM phosphate, 80 mM KCl, 20 mM NaCl, pH 7.0), heating at 95 C for 5 min, and then cooling on ice

Mutat Res 2008;640:174179
