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Crystallisation To obtain peptide-free DAP BII crystals 39 , the samples were crystallised using the hanging-drop method, in which 1 l of protein solution (10 mg/ml DAP BII in 80 mM Tris-HCl, pH 8.5) was mixed with the same volume of reservoir solution (18% (w/v) PEG8000, 20% (v/v) glycerol and 2 mM zinc chloride in 0.08 M CHES-NaOH, pH 9.5) and incubated at 293 K
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[DOI] [PubMed] [Google Scholar] 15.Pyra M, Casimiro I, Rusie L, et al
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