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Exploring the role of B vitamins in liver health Non-alcoholic fatty liver disease is a significant public health concern, closely tied to obesity and metabolic disorders

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The age-related decline in melatonin and dampening of circadian rhythms likely contributes to sleep problems, metabolic and cognitive decline, and increased vulnerability to age-related diseases

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Glutathione was measured using the Glutathione Colorimetric Detection kit (Invitrogen, EIAGSHC) according to protocol and detected with a Synergy2 plate reader (BioTek)