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A new highly thyrotropin receptor-selective small-molecule antagonist with potential for the treatment of Graves orbitopathy

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TR146 cell culture models are useful for the mechanism but should be complemented by ex vivo porcine buccal tissue under flow or tilt to improve validity

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10.1080/13880209.2022.2070220 52 MuroP.ZhangL.LiS.ZhaoZ.JinT.MaoF.et al (2024)

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The supernatant (cytosolic fraction) was then collected and the organellar pellet washed twice and resuspended in 100 L of 1% Triton X-100 (VWR, 0694) in 50 mM Tris-HCl and 150 mM NaCl, pH 7.4 to lyse the mitochondrial membrane

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