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Description
A new highly thyrotropin receptor-selective small-molecule antagonist with potential for the treatment of Graves orbitopathy
TR146 cell culture models are useful for the mechanism but should be complemented by ex vivo porcine buccal tissue under flow or tilt to improve validity
10.1080/13880209.2022.2070220 52 MuroP.ZhangL.LiS.ZhaoZ.JinT.MaoF.et al (2024)
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The supernatant (cytosolic fraction) was then collected and the organellar pellet washed twice and resuspended in 100 L of 1% Triton X-100 (VWR, 0694) in 50 mM Tris-HCl and 150 mM NaCl, pH 7.4 to lyse the mitochondrial membrane
