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Fluorescence detection of reactive oxygen species (ROS and Superoxide) Intracellular ROS levels were measured using the ROS-ID Total ROS/Superoxide Detection Kit (ENZ-51010, Enzo Life Sciences, NY, USA) following the manufacturers instructions

The gut microbiome is a huge and complex system that regulates homeostasis mostly through the metabolites it produces (Li et al., 2014)

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Funding Statement This work was supported by the National Natural Science Foundation of China (82170299)

GenBank AA305138 EST176158 Colon carcinoma (Caco-2) cell line II Homo sapiens cDNA 5' end similar to ras-like protein TC21, mRNA sequence

This strategy provides a highly reliable and cost-effective alternative to both conventional external calibration and isotope dilution techniques, achieving high precision ( 5%) and accuracy ( 15%) even for heavily matrix-suppressed analytes