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In a double-blind, placebo-controlled trial in human, healthy adults given a single 12 g oral dose of Gotu Kola showed a significant drop in acoustic startle reflex amplitude at 30 and 60 minutesindicating a rapid anxiolytic effectwithout changes in self-reported mood, heart rate, or blood pressure [19] In a 2016 study, mice subjected to 72 hours of sleep deprivationa common model for stress-induced anxietywere treated with Gotu Kola extract at doses of 150 and 300 mg/kg for 8 days

This metabolism-immunity crosstalk disrupts both innate and adaptive immunity: it drives the aberrant activation of macrophages and neutrophils while reprogramming T cell metabolismenhancing glycolysis in pro-inflammatory Th1/Th17 cells and impairing mitochondrial oxidative phosphorylation in Tregsultimately establishing a chronic intra-renal inflammatory microenvironment that accelerates glomerulosclerosis and tubulointerstitial fibrosis

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