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The amino acids at those positions are likely buried in the protein's interior
A weight of -1 was assigned to genes (or proteins) that inhibit the direction of signal transmission in the pathway to reflect the inhibitory effect
The Cancer Genome Atlas (TCGA) contains enough data that validates on copy number alterations (CNA), mutational profiles, mRNA expression and microRNA (miRNA) expression
10.1186/bcr2208 72
The mean CT values for each sample were used to calculate relative expression using a variation of the 2^(- delta delta CT) method 36
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