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This effect may be attributed to a marked reduction in the expression of MuRF1 and atrogin-1, thereby delaying age-dependent dysregulation of muscle mass through the modulation of genes involved in muscle regeneration and catabolism (66)

The digested peptides were desalted with C18 solid-phase extraction, concentrations were determined by BCA, and samples were stored at -20C until being subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS)

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