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Commonly, one joint is affected by a gout flare, but it can still affect multiple joints at a time

2009;297(3):E620E628

[DOI] [Google Scholar] 240.Wjcik K, yszczarz A, Kolarzyk E

Evaluation of different types of arsenic methylation and its relationship with metabolic syndrome in an area chronically exposed to arsenic

At this stage of purification GST-X fusion should be clean and depending on the nature of furhter experiments that we want to perform, we can either elute the fusion with glutathione (excess of the glutathione will compete and displace GST-X protein from the beads) or cleave the GST tag and release protein X (see cartoon below)

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