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The endogenous opioid peptides are generated from four precursors: proopiomelanocortin, proenkephalin, prodynorphin, and pronociceptin/orphanin FQ (Nakanishi et al., 1979

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This VDF cocktail induced high expression of key hepatic markers such as AFP, HNF4, and Albumin (ALB), with an efficiency of 95.5% (HNF4a+/AFP+ positive cells), closely matching that of the growth factor-based control

any temperature excursion above 8C causes irreversible peptide denaturation

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